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By measuring basal (no antibody) and maximally stimulated (4,600 pg/mL LPS) responses in the same blood sample, the EA of the test specimen is calculated by integrating chemiluminescence over time.
The NLR of subsequent postoperative days was not calculated because routine blood specimens were not collected beyond postoperative day 1 in all the patients included in the analysis.
Patient blood specimens were used without spiking.
Blood specimens were held at RT (20 25 °C) and were processed as soon as possible.
All healthy donor and patient blood specimens were acquired and handled according to the protocols approved by the Massachusetts General Hospital MGH Institutional Review Boardrd.
Peripheral blood specimens were sampled at serial time points (indicated by arrows), and CLL cells underwent whole-exome sequencing.
Blood specimens were collected in EDTA tubes.
Blood specimens were tested only by blood culture.
Peripheral blood specimens were collected from the caudal vena cava.
Blood specimens were collected before intervention.
All blood specimens were stored at −80°C.
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