Sentence examples for blood specimens using a from inspiring English sources
DNA was isolated from blood specimens, using a modification of the Qiagen QIAmp 96 DNA Blood Kit (Qiagen GmbH, Hilden, Germany, http://www.qiagen.com).
DNA was extracted from blood specimens using a commercial purification system (Wizard Genomic DNA Purification Kit, Promega, Madison, WI) according to the manufacturer's instructions for DNA purification from blood.
For the SARI program a case of bacteremic pneumococcal pneumonia (BPP) was defined as the identification of S. pneumoniae in blood specimens using a single-target (lytA) quantitative real-time PCR assay adapted from Carvalho et al. [ 16]. lytA-positive specimens (cycle threshold (Ct -value < 40) were serotyped by real-time PCt -valuean adaption of the method described by Azzari et al. [ 17].
Fetal genomic DNA was extracted from 5-mL amniotic fluid volumes or 3-mL cerebrospinal fluid volumes, and neonatal DNA was extracted from 200 μL of umbilical cord blood specimens, using a High Pure PCR Template Preparation Kit (Roche™, Mannheim, Germany).
Genomic deoxyribonucleic acid (gDNA) was isolated from patients' whole-blood specimens using a QIAsymphony DNA kit (Qiagen, Valencia, CA).
CD34+ cells, which constitute approximately 1% of nucleated blood cells in umbilical cord blood [28], were isolated from the cord blood specimen using an immunomagnetic separation technique, as previously described [35].
Serum triglyceride, HDL, and fasting plasma glucose were measured with fasting blood specimens using, respectively, a glycerol removal method, an enzymatic method, and a stepwise high-performance liquid chromatography method.
Unlike our findings, Matsuda and co-workers reported excellent sensitivity of qPCR compared to culture for detection of these bacteria from blood specimens using these primers.
