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Genomic DNA was prepared from whole blood specimens using standard procedures.
Unlike our findings, Matsuda and co-workers reported excellent sensitivity of qPCR compared to culture for detection of these bacteria from blood specimens using these primers.
DNA was isolated from blood specimens using standard methods.
The DNA was isolated from blood specimens using the QIAamp DNA Blood Midi Kit (Qiagen).
DNA was prepared from tissue and blood specimens using the conventional proteinase K digestion and phenol/chloroform extraction method.
DNA was isolated from blood specimens, using a modification of the Qiagen QIAmp 96 DNA Blood Kit (Qiagen GmbH, Hilden, Germany, http://www.qiagen.com).
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Blood specimens used to derive lipids and HbA1c were processed by Quest Diagnostics Laboratories.
Five milliliters of whole blood was drawn from each patient by venipuncture; blood specimens used for the preparation of serum were collected in a serum separator tube with clot activator and gel.
As referred to in Early Dengue infection and outcome study (EDEN) – study design and preliminary findings, Ann Acad Med Singapore 35 (11), 783-789 (2006), the blood specimens used in this study were obtained from the adult dengue cohort in Singapore.
The serovar specific loci were present in all available genome sequences and we ensured the presence of the target sequences on DNA extracted from 140 S. Typhi and S. Paratyphi A strains by PCR (these strains included the 100 strains isolated from blood specimens used in later experiments).
CD34+ cells, which constitute approximately 1% of nucleated blood cells in umbilical cord blood [28], were isolated from the cord blood specimen using an immunomagnetic separation technique, as previously described [35].
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