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For the MDP feasibility study, the Abbot IMX ELISA test (Abbot Diagnostics, Africa Division), in combination with the Vironostika HIV1/2 ELISA for positive and equivocal results, was used on whole blood sourced from venepuncture.
For the MIRA and HPTN 055 studies, HIV diagnostic testing was achieved using two rapid tests on whole blood sourced from either finger-prick or venepuncture (Determine HIV-1/2, Abbot Laboratories, Tokyo, Japan and Oraquick, Orasure Technologies, Bethlehem, PA, USA).
For the MIRA and HPTN 055 trials, HIV diagnostic testing was achieved using two rapid tests on whole blood sourced from either finger-prick or venepuncture (Determine HIV-1/2 Abbott Laboratories, Tokyo, Japan and Oraquick Orasure Technologies, Bethlehem, PA, USA).
The Abbott IMX ELISA test (Abbott Diagnostics, Johannesburg, South Africa) in combination with the Vironostika HIV Uni-form II plus O ELISA (previously Organon Teknika, USA, currently bioMérieux, France) for positive and equivocal results was used on whole blood sourced from venepuncture for HIV diagnosis during the MDP Feasibility Study.
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Our results indicate that mean acute-phase protein concentrations differ according to pyometra type/severity and blood source and suggest the possible use of peripheral blood levels of CRP, SAA, and Hp to monitor inflammation during the course of pyometra.
The blood source represents only 17% of the residuals' variance suggesting that variability around model predictions originates from noise in the experiment rather than infected blood from the same volunteer consistently over- or under-performing (paired rank sum test, p-value = 0.83).
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