Blood sampling was started exactly from day 15th post delivery that continued for a period of six months with an interval of 15 days between two successive samplings.
The syringe pump for extraction of the arterial reference blood sample was started with a speed of 0.5 mL/min and continued over 2 min. The microspheres were injected 0.5 min after starting the reference blood sampling with a rate of 1 mL/min.
To evaluate continuous dosing of sorafenib combined with single-dose sirolimus, blood samples were collected starting on day 16, when patients were already treated with sorafenib since day 5 and single-dose sirolimus was added.
Blood samples were taken before starting these experiments, at day 0 and 4 days after cell transplantation, and at autopsy (day 92) in order to determine the proportion of NK cells in the blood by flow cytometry.
Blood samples were taken before starting on AZA, and TPMT activity was measured.
Nonfasting blood samples were obtained, and starting in 1995 when funding became available, A1C was measured on fresh EDTA blood samples using high-performance liquid chromatography (Diamat automated glycated hemoglobin analyzer; Bio-Rad, Hemel Hempstead, U.K).
Blood samples were drawn before starting DAA infusion, after 4, 8 and 12 hours (at the end of the infusion period), and on treatment days 2, 3, 4, 5 and 6.
In addition, one patient in the liraglutide group was excluded from the pharmacodynamic analysis due to a protocol deviation (premeal blood sampling was delayed until after the start of the meal).
At the first bite a timer was started and additional blood samples were taken at 15, 30, 45, 60, 90 and 120 min after the start of the study meal.
Blood sampling was performed by tail bleeding before start of sensitizations and afterwards weekly as indicated in Fig. S1a.
