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The VO2peak test and blood sampling were repeated 2 days (2d), 1 week (1w), 2 weeks (2w), and 4 weeks (4w) after each of the three blood donations (see below) following exactly the same conditions (Fig. 2).
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The blood sampling was repeated after one year of supplementation.
To verify the appropriateness to discriminate cells staining positive for both CD4 and CD25 and negative for CD127 as Tregs, blood sampling was repeated for seven newly enrolled patients; two healthy volunteers, two patients with flu-like syndrome and three patients with H1N1 infection.
Blood sampling was repeated 3 hours after treatment.
In 367 patients, blood sampling was repeated on days 3, 7, and 10.
Blood sampling was repeated within the first 24 hours upon advent of sepsis.
At the end of study at week 12, blood sampling was repeated.
For 83 patients, blood sampling was repeated on the day of worsening of sepsis stage.
During the 4 h feeding period, the subject or patient continued to rest in the supine position and energy expenditure measurements were repeated every 40 min for 20 min and blood sampling was repeated every 30 min.
One week later, when the administered drug was supposed to have been removed from the blood, the whole study protocol including MMF administration and blood sampling was repeated (Supplementary Figure 1).
Patients underwent ahyperoxygenation test (5 minutes of FiO2 100%%), and arterial and central venous blood samples were repeated.
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CEO of Professional Science Editing for Scientists @ prosciediting.com