Data from the A3243G mutation taken from blood samples were adjusted to correct for the known decrease in the A3243G mutation level in blood with age (Rajasimha et al., 2008).
Blood samples were adjusted with APC to final concentrations of 0.045 μg/ml APC (group 1, therapeutic dose) and 0.225 μg/ml APC (group 2, fivefold therapeutic dose), respectively.
After measuring the morning cortisol level, blood samples were adjusted with hydrocortisone (Pharmacia, Erlangen, Germany) to final concentrations of 4.5 μg/ml (group 1), 9 μg/ml (group 2, low dose) and 90 μg/ml (group 3), respectively.
Blood samples were adjusted with glucose (Sigma, Taufkirchen, Germany) to final concentrations of 5 mmol/l (control group), 10 mmol/l (group 1) and 15 mmol/l (group 2), respectively.
In Brazil, random blood samples were taken, and values were adjusted for time since the last meal (18).
So the blood sampling time was adjusted in multiple regression analysis.
The association between 25-OHD and death rate, adjusted for sex, age at diagnosis, and season of blood sampling, is shown in Model II.
Furthermore, studies in which blood samples were collected either before treatment or ≥7 days after the last chemotherapy cycle demonstrated significantly lower sensitivity (adjusted P = 0.025) but higher specificity (adjusted P < 0.001) compared with those with other timeframes.
Blood samples were drawn.
