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All blood samples were spotted on DBS cards.
For PCR analysis, blood samples were spotted onto Whatman 3MM filter paper.
Individual blood samples were spotted onto Whatman No. 4 filter papers and kept dry at room temperature until further processing.
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All samples were spotted in duplicate.
Blood samples were also spotted on Whatman 3MM filter paper for parasite DNA extraction.
Blood samples were collected by a dry blood spot fingerstick technique and tested for HSV-2 and HIV 1/2 antibodies on all participants using standard methods for detecting HSV-2 (HerpeSelect 2 ELISA IgG, Focus Diagnostics, CA) and HIV 1/2 (Vironostika HIV Uniform II AG/AB, bioMérieux, France) (Murex HIV; Murex Biotech) (HIV Tridot; J. Mitra, New Delhi, India) following pre-test counseling.
Therefore, single spot urine and blood samples were collected during scheduled appointments instead of at a standardized time during the day.
Spot urine- and blood samples were collected at gestational weeks 12, 20, 28, 32, and 36.
Blood samples were used to prepare blood smears, filter paper spots or to separate red blood cells (RBCs).
Each visit, 24-h urine, spot morning urine, and blood samples were collected.
Whole blood samples were obtained from all patients, and buccal swabs and blood spots were collected from healthy controls.
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