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For the purpose of analyzing the T-cell compartment, in particular CD95 expression on naive T cells, additional blood samples were specifically collected from healthy volunteers not following the labeling protocol after having provided written informed consent.
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Specifically, blood samples were fixed and incubated with peridinin-chlorophyll protein-linked (PerCP -anti-CD61 antibodies (BD Biosciences), fluorescein isothiocyanate-linked (FITC)-anti-fibrinogen antibodies (Dako), and PerCP -anti-CD61nked (PerCP -anti-CD61ntibodies (BD Biosciences).
Blood samples were taken to investigate biomarkers of genotoxicity, specifically, primary DNA damage as damage index (DI), tail length and% of tail DNA using the Comet assay (alkaline version) and chromosome damage as micronucleus (MN) frequency in lymphocytes.
Blood samples were collected at this time by a phlebotomist hired specifically for this study.
All blood samples were taken during routine health checks by experienced veterinarians and not specifically for this study.
Blood samples were obtained from non-fasting participants, and a dedicated sample was collected specifically for the purpose of the primary study [ 21].
Blood samples were drawn.
Three blood samples were taken.
Blood samples were obtained preoperatively.
Blood samples were taken for CD4 counts.
All blood samples were drawn into EDTA blood tubes.
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