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Blood samples were smeared on glass slides, and inclusions in reticulocytes were visualized by staining with new methylene blue.
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In the curative test, blood samples were taken and smears prepared daily to evaluate the curative ability of the extract.
Each sample was smeared onto glass slides.
After clinical assessment and confirmation of the diagnosis from thick and thin blood smears, baseline blood samples were taken for routine hematology and biochemistry.
Blood samples were used to prepare blood smears, filter paper spots or to separate red blood cells (RBCs).
When sufficient parasitaemia was visible in giemsa stained thin blood smears, blood samples were taken and DNA extracted (following [ 37]) for PCR and sequencing.
Blister packs were requested for a pill count, and blood samples were collected for a blood smear and a malaria rapid diagnostic test (mRDT) (Pf-specific from ICT Diagnostics, Cape Town, South Africa).
At indicated times, peripheral blood samples were obtained from the mouse tail vein and smeared onto microscope slides.
EDTA-blood and whole blood samples were collected on July 30 and a blood smear were prepared and stained with May-Grünwald Giemsa. A. phagocytophilum inclusions were detected by light microscopy in 34% of the neutrophils.
Blood samples were collected from all 21 animals, following which blood smears were prepared, subjected to quick Romanowsky staining and examined under the optical microscope.
The collected blood samples were utilized immediately for the preparation of thin blood smears and were then kept at −20°C until DNA extraction.
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