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Healthy human blood samples were procured from the Indiana Blood Center (Indianapolis, IN).
Lion and Tiger blood samples were procured with due approval of the competent authorities of the States and Union Government of India.
Blood samples were procured at each evaluation visit, except for days 21 and 22, and skin biopsies were collected on days 0, 7 (single doses only), and 28 (multiple doses only).
For analysis in plasma samples blood samples were procured from healthy volunteers and concentrations of piracetam and levetiracetam in plasma were analyzed by the proposed method. 2 mL of plasma was mixed with 2 mL of acetonitrile and 2 mL of drug solution was shaken, vortexed for 5 min, and centrifuged at 3000 rpm for 10 min.
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Cattle blood sample was procured from the owner of the animal.
Two ml of heparinised blood sample was procured for arterial blood gas (ABG) analysis from the radial artery and was transported to the laboratory immediately for processing.
The bone samples were procured by drilling.
Seed samples were procured from Indian Agro centre, Arakkonam, Vellore to raise plants for the experiments.
Samples were procured from conveniently sampled fruit and vegetable farmers around Hachaanga and St. Mary's areas of Monze.
All samples were procured immediately before or during their endoscopy.
ESCC tissue samples were procured from Chinese Academy of Medical Sciences Cancer Hospital.
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