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Blood samples were periodically taken for antibody titration and specificity analyses by ELISA or western blot.
Arterial blood samples were periodically removed for blood gas analysis.
Glucose (8.99 mg/min, i.v). was infused, and blood samples were periodically withdrawn for the determination of glucose (using glucose sensors, Ascensia® ELITE XL, Bayer), and insulin (RIA, RI-13K®, Linco, USA).
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Samples were periodically withdrawn to run the activity assay.
Samples were periodically analyzed by western-blotting.
Population samples were periodically stored −80 °C.
Samples were periodically taken for the measurements of OD600, residual glucose concentration, and L-ornithine concentration.
Additional blood samples were collected periodically during the washout period (14, 28, 42, 54, and 63 days after the last dose of aleglitazar).
On the first day of reFSH/saline treatment, blood samples were collected periodically from 1 h prior to treatment to 6 h post-injection via an indwelling jugular catheter to determine acute changes in FSH concentrations.
Venous blood samples were taken periodically over the course of the PET scan.
Matched breath and blood samples were collected periodically during the experiment.
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