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The blood samples were labeled anonymously and could not be traced back to a specific donor.
For each patient, EDTA blood samples were labeled with CD34/45+ and IgG1/CD45.
Blood samples were labeled with the following monoclonal antibodies: PE-conjugated anti-IL21R, APC-conjugated anti-CD3, PerCP-conjugated anti-CD4, FITC-conjugated anti-CD19 (BD Biosciences, Amsterdam, The Netherlands) for 15 minutes in the dark.
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The blood samples were labelled by number only and ordered randomly within each case control pair.
Questionnaires, informed consents, clinical forms and blood samples were labelled using alphanumeric codes to identify the site and participant.
The blood samples were labelled with a bar code, gently mixed and stored frozen upright in a -20 degree centigrade freezer pending DNA extraction.
In the following the parameters obtained with the IF from blood samples are labelled with sample and those using the image-derived IFs are labelled with img.
Samples were labeled with a unique identifier.
Blood, fecal, saliva, and urine samples are labeled with preprinted bar-code labels at the time of collection and transported to hub laboratories.
The blood samples were randomly labeled and stored until processing at 4°C (randomization and blinding).
All samples are labelled with their intended metals composition.
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