Blood samples were kept at 4 °C throughout the procedure.
Blood samples were kept in total darkness until clotting at room temperature to avoid light induced MEL degradation.
Blood samples were kept in total darkness before being centrifuged, then serum samples were placed into −80 °C until MT measurement was performed.
In order to avoid the non-specific rise of mHLA-DR over time [9], blood samples were kept on ice and measurements were performed every 25 min (i.e., turnaround time on Accellix) during 3 h after sampling (i.e., n = 6 measurements [10]).
Blood samples were kept on ice during plasma collection.
Blood samples were kept on ice until fractionation.
Blood samples were kept at 4°C for 1 h and then centrifuged.
Blood samples were kept under refrigeration in an ice box (at approximately 15°C) for about 15 minutes, taken to the laboratory and used to feed A. aquasalis.
Blood samples were kept on ice for a maximum of 1 3 hrs before centrifugation, at which point plasma was separated and stored at −80°C for 3 months prior to carotenoid analyses.
Blood samples were kept on ice for no longer than 6 h after bleeding (venipuncture) and then aliquoted into whole blood, plasma and pellet and immediately stored at −80°C.
Blood samples were kept on ice.
