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Upon collection, blood samples were instantly chilled on ice and centrifuged (2,400 g, 15 min, 5°C) within 30 min. Plasma were stored at −20°C until analysis.
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The blood samples were immediately centrifuged at 2500 g for 15 min to obtain serum which was instantly cooled down, stored at −80 centigrade.
The CA samples were instantly frozen and transferred to the analysis chamber with 2 4×10−7 Pa base-vacuum, −150°C.
All samples were instantly frozen in liquid nitrogen and stored at -80°C until use.
Obtained samples were instantly frozen in liquid nitrogen and stored at −80°C.
The fresh blood samples were processed instantly for PBM isolation by experienced technicians.
Blood samples were analyzed immediately, without freezing.
On admission, arterial blood samples were collected from arterial or femoral artery and parameters for the assessment of acid base status were instantly measured.
Blood samples were drawn.
Three blood samples were taken.
Blood samples were obtained preoperatively.
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