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Peripheral blood samples were enriched for cells expressing the epithelial-cell adhesion molecule (EPCAM) with antibody-coated magnetic beads, and cells were labeled with the fluorescent nucleic acid dye 4,2-diamidino-2-phenylindole dihydrochloride.
Peripheral blood samples were enriched with the CellSearch System with ferrofluid nanoparticles coated with anti-EpCAM antibody.
Blood was collected using the Vacutainer CPT Cell Preparation Tube System (BD, Heidelberg, Germany) and the blood samples were enriched in monocytes by negative selection, using RosetteSep Monocyte Enrichment Cocktail (StemCell Technologies, Vancouver, Canada).
Using the optimised broth Salmonella Typhi bacteria in artificial blood samples were enriched in blood culture and then detected by a PCR targeting the fliC-d gene of Salmonella Typhi.
Fresh blood samples were enriched by applying B-RosetteSep (StemCell Technologies, Vancouver, Canada) to aggregate unwanted cells with erythrocytes and Ficoll-Hypaque (Seromed, Berlin, Germany) density gradient purification resulting in purity > 98% of CD19+/CD5+ CLL cells.
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The goby samples were enriched and sequenced for the same 4,434 loci.
The inoculated samples were enriched for 24 and 48 h.
Acidic samples were enriched in acetic acid and formic acid, while neutral/basic samples were enriched in acetate and formate.
Remaining remission samples were enriched in pools of 10 samples.
Samples were enriched for CD34+ and cultured as described.
Our study of 3663 unpurified blood samples was not enriched for samples that had previously generated imbalanced peak data in MALDI-ToF analyses.
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