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Non-targeted mass spectrometry based analyses of the patients' blood samples were done by Metabolon similarly to the endotoxemia study.
Methylation studies of DNA from blood samples were done using the Infinium Human Methylation 450K BeadChip assay (Illumina) according to the manufacturer's instructions.
The 'control'-group had to sit on a chair for 5 min and afterwards to lay on a stretcher for 15 min. The blood samples were done at equivalent times.
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Whole exome sequencing of DNA extracted from fresh frozen tumors and matched normal blood samples was done as previously described8, 26.
Ultra-trace detection of estradiol valerate (EV) in whole blood samples was done by coupling Fast Fourier transform square wave voltammetry (FFTSWV) and electromembrane extraction (EME).
A second collection of blood samples was done for measurement of homocysteine, folate, B6 and B12 concentrations.
Bacterial enumeration in tissue homogenates or in blood samples was done by plating serial dilutions in PBS on BHIA and calculating cfu/organ or cfu/1 ml blood.
Bacterial enumeration in tissue homogenates or in blood samples was done by plating serial dilutions in PBS on BHI agar and calculating the cfu/organ or cfu/1 ml blood.
Detection of EHV-1 and EHV-4 genes in the blood samples was done using polymerase chain reaction (PCR).
Extraction and purification of RNA from venous and capillary blood samples was done according to the manufacturer's instructions with an additional second wash step using AW2 buffer.
Microscopic examination of blood samples was done after staining the prepared thin blood smears with Giemsa as per standard protocol [ 19] and examined under oil immersion objective of the microscope to detect the piroplasms and the results obtained were compared to that of PCR assay.
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