Blood samples were allowed to clot O/N at 4 °C before centrifuging for 20 min at 2000×g.
The blood samples were allowed to clot at room temperature.
Blood samples were allowed to clot and the serum was collected and stored frozen at −80°C.
Blood samples were allowed to clot at 4°C, centrifuged and stored at −80°C until assayed.
Blood samples were allowed to clot, centrifuged at 2000×g for 20 minutes, and then stored at −80°C for creatine kinase measurement and immunoblotting.
Blood samples were allowed to stay at room temperature for a maximum of 6 hours before being processed in a laboratory.
All blood samples were allowed to cloth at room temperature for 1 hour, spun at 4000 rpm for 20 min at 4°C to obtain serum and stored at −80°C until used.
The blood samples were allowed to clot at room temperature for 1 h, placed at 4°C overnight, and the sera pooled by centrifugation at 200 g for 10 min. Total muscle proteins (TMPs) were prepared by the method of Chen and Resh [45].
Immediately after collection, fasting blood samples were allowed to clot at room temperature for four hours, and the serum were collected and centrifugated at 3000 rpm/min for 15 min. Before pooling the samples, the protein concentration of the serum samples was determined by Bradford assay on a Microplate Reader (Bio-Rad, Model 680).
Trunk blood samples were allowed to clot at room temperature.
Blood samples were allowed to clot before antibody assays were run.
