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Testosterone in blood samples was quantified by RIA (Beckman-Coulter, Villepinte, France).
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Blood samples were quantified for hTERT mRNA expression using real-time polymerase chain reaction.
Purified DNA from each blood sample was quantified by spectrophotometry, yielding 20 32 ng/μL of DNA.
The presence of CTC in each blood sample was quantified by fluorescence microscopy from 10 images of representative areas (magnification × 100) that were acquired as described above.
RNA samples were quantified using spectrophotometry.
All samples are quantified.
Samples were quantified in triplicate.
Samples were quantified using a Bradford assay.
a minimum of 6 samples were quantified.
Samples were quantified using peak area ratios.
Samples were quantified using a PhosporImager (Molecular Dynamics).
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