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Subjects were required to submit overnight fasting blood samples utilized for the different metabolic parameters.
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Genomic DNA was extracted from whole blood samples, utilizing the salting out method.
For blood samples, we utilized the tool Cn.
Their blood samples were utilized for ICC as confocal control specimens but not were used for FCM.
The collected blood samples were utilized immediately for the preparation of thin blood smears and were then kept at −20°C until DNA extraction.
IGRAs differ from each other mainly with respect to the technique of IFN-γ detection (enzyme linked immunospot; ELISPOT vs. enzyme linked immunosorbent assay; ELISA) and the samples utilized (peripheral blood mononuclear cells vs. whole blood).
While in those with regular menstrual cycles, blood samples were obtained during the follicular phase of the cycle, except for samples utilized for the assay of progesterone that performed in the day 21 of the cycle (luteal phase).
Only endogenous 25(OH)D2 and/or 25(OH)D3 contained in human blood samples should be utilized for this purpose.
This secondary analysis utilized blood samples and cognitive scores (learning, memory, executive function, verbal fluency, and processing speed) obtained at baseline and three-month follow-up of the RCT.
In our study, we have utilized blood samples collected from stroke patients within 6 18 months in time scale from the index stroke.
The difference in our study is firstly that our prospective study utilized blood samples that did not come from the same patients as our tumour samples.
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