Lactate concentrations were obtained at rest for all experimental subjects, and immediately following the fourth repetition, via capillary blood samples using the LactatePro analyzer.
The DNAs of 49 samples were isolated from 4 mL of frozen blood samples using the Gentra Puragene Blood Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.
We employed blood samples using the same sample tubes collected at one-point arterial blood sampling for the brain QSPECT DTARG method.
DNA was extracted, within 3 days, from 300 µl whole blood samples using the Wizard Genomic DNA Purification kit (Promega, USA) according to the manufacturer's instructions.
DNA was extracted from blood samples using the QIAamp DNA Blood Mini Kit (Qiagen, Valencia, CA).
Genomic DNA was isolated from blood samples using the Puregene® DNA purification kit (Gentra, Minneapolis, MN) following the manufacturer's guidelines.
Genomic DNA was isolated from blood samples using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA).
DNA was extracted from the modern Emu blood samples using the former kit and in accordance with the manufacturer's instructions.
DNA was isolated from maternal and fetal blood samples using the Autopure automated system (Gentra Systems (Minneapolis, MN)).
Genomic DNA was isolated from whole blood samples using the PureGene DNA Blood Kit (Gentra, Minneapolis, MN, USA).
Genomic DNA was prepared from peripheral blood samples using the Puregene DNA purification kit (Gentra, Minneapolis MN, USA).
