We handled, centrifuged, and stored blood samples using established protocols [28].
We prepared DNA from peripheral blood samples using standard protocols in order to avoid artifacts related to transformation and cell culture.
Genomic DNA was isolated from peripheral blood lymphocytes from 10 ml blood samples using the protocol described by Miller et al [ 25].
DNA was extracted from paired placenta and cord blood samples using standard kits and protocols (DNeasy Blood & Tissue Kit, Qiagen, UK).
Genomic DNA was obtained from peripheral blood samples using standard phenol-chloroform extraction protocols (29).
DNA was extracted from EDTA venous blood samples using a standard sucrose lysis protocol.
We isolated DNA from blood samples using a high-salt extraction protocol following Bruford et al. [ 42].
Genomic DNA from PJS patients was isolated from EDTA venous blood samples using a standard sucrose lysis protocol.
WES was performed for 38 tumor samples from 18 patients and matched blood samples using the SureSelect Human All Exon Kit Agilent Technologiess) according to the manufacturer's protocols, as previously described (Supplementary Table S1 31,34,35.
Total leukocyte RNA was extracted from the blood samples using TRIzol (Invitrogen), according to the manufacturer's protocol.
