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CRP was measured (mg/L) during 2006 2011 in fresh blood samples using high-sensitivity methods.
CRP was measured (mg/L) at the third health examination (2006 2011) in fresh blood samples using high-sensitivity methods using the Siemens Dimension clinical chemistry analyser (Newark, Delaware, USA), with between-batch coefficient of variation (CV) values of 3.1% at 7.86 mg/L and 3.7% at 88.75 mg/L.
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Glycosylated haemoglobin (HbA1c) was measured on fresh EDTA blood samples using high-performance liquid chromatography.
Plasma was prepared from the collected blood samples and a select number of circulating inflammatory proteins were measured using high-sensitivity enzyme-linked immunosorbent assay (ELISA) kits that were commercially available.
Fasting venous blood samples were taken for the analysis of C-reactive protein (CRP), which was performed using high-sensitivity ELISA (R&D Systems, Oxford, UK), and total and high-density lipoprotein (HDL) cholesterol and triglycerides, which were measured within 72 h in serum stored at 4°C using enzymatic colorimetric methods.
High molecular-weight DNA was obtained from blood samples using classical DNA extraction procedure [ 17].
In order to improve microarray sensitivity, globin RNA species were removed from blood samples using the GLOBINclear® kit (Ambion, Austin, TX, USA).
Plasma samples were obtained after centrifugation of arterial blood and analyzed using high-performance liquid chromatography for metabolites.
Polymerase chain reaction testing (PCR) of peripheral blood samples has a high sensitivity and specificity for the diagnosis [24].
A1C was measured from blood samples collected at each visit using high-pressure liquid chromatography.
Blood samples for lipids, glucose, and high-sensitivity CRP measurements were taken in the morning after fasting at least 12 hours.
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