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Platelets-monocytes and platelets-neutrophils aggregates were analyzed in citrated whole blood samples using an antibody panel, including anti-CD45-V500, anti-CD14-APC (monocytes), anti-CD15-PE (neutrophils), and anti-CD61-PerCP.
Genomic DNA was isolated from EDTA-preserved blood samples using an automated DNA isolation system NA-30000) (KurabOsaka, Japanpan).
CD4+ lymphocyte counts were measured on EDTA-anti-coagulated whole blood samples using an EPICS Profile II flow cytometer (Coulter Corporation, Hialeah, Florida) [ 8].
Full blood counts for the determination of RBC mean cell volumes were performed on fresh EDTA-anticoagulated blood samples using an MDII-18 counter (Beckman-Coulter, Fullerton CA, USA).
Haematological analysis included the determination of PCV, total leukocyte count, and the concentrations of fibrinogen and total protein in EDTA blood samples using an automated blood analyzer (CELL-Dyn 3500, Abbott Diagnostics Division, Baar).
DNA was purified from blood samples using an extraction method described below that yielded high-quality DNA (high purity with respect to proteins and salt, optical density (OD) 260/280 and 260/230 with rate values ≥ 1.8) and decreased the effect of PCR inhibitors such as hemoglobin and some components of the culture medium.
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DNA was isolated from blood samples using a salting-out method (after Lahiri and Schnabel, 1993).
Then, 100 ng of total RNA was used to quantify expression levels of miRNAs from animal blood samples using a probe-based miRNA expression assay (NanoString Technologies, USA).
To develop a simple, rapid, sensitive and affordable assay method for the determination of glucose in blood samples using a novel approach.
Genomic DNA was extracted from peripheral blood samples using a salting out method as described by Miller et al. [25].
A hundred blood samples using a fixed type of sample tubes and a fixed radionuclide may be required to set up the robust conversion function.
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