Genomic DNA was extracted from peripheral blood samples using a salting out method as described by Miller et al. [25].
A hundred blood samples using a fixed type of sample tubes and a fixed radionuclide may be required to set up the robust conversion function.
To develop a simple, rapid, sensitive and affordable assay method for the determination of glucose in blood samples using a novel approach.
A hundred blood samples using a fixed type of sample tube and a fixed radionuclide may be required to set up the conversion function and determine the simple error.
Blood glucose levels were determined on tail vain blood samples using a Precision Xtra glucometer in resting mice and within 30 seconds of exhaustion (Abbott Labs).
The Cy5.5 signal could be readily detected and quantified in small volume blood samples using a 96 well format NIR fluorescence scanner as described in the Methods section.
Blood lactate concentrations were determined on tail vain blood samples using a Lactate Pro blood lactate test meter (Arkray USA) in resting mice and within 30 seconds of exhaustion.
The presence of each of the four human malaria species was assessed in all blood samples using a semi-quantitative post-PCR, ligase detection reaction/fluorescent microsphere assay (LDR-FMA)[30].
Human TSST-1 IgG antibodies were determined from the blood samples using a sandwich ELISA method.
PBMCs were isolated from a total of 12 blood samples using a Histopaque-1077 solution.
Lymphocyte counts for all subjects were determined in peripheral blood samples, using a differential blood count.
