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Prior to initiation of plasma collection for the MA.14 trial, in a pilot study to assess the potential effect of this collection/storage process, we assessed OPN plasma levels from eight blood samples maintained at room temperature for 4 vs. 24, 48 or 72 hours, and found that measured values did not differ with time at room temperature.
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Blood samples were maintained at room temperature and all samples were assayed within 30 min of collection.
Blood samples were maintained at room temperature for different time intervals and processed within a maximum of 72 hours after blood drawing.
Blood samples were maintained at 4°C until their processing.
Heparin whole blood samples were maintained at room temperature for up to 12 hours prior processing.
Blood samples were maintained at room temperature and assayed within 30 minutes of collection.
The blood samples were maintained at room temperature and processed within a maximum of 72 h after collection.
The blood samples were maintained at room temperature for at least 30 min and then centrifuged at 1300 ×g for 15 min at 4°C.
Each blood sample was maintained at 4°C and centrifuged, and plasma was distributed in aliquots and stored at −80°C until measurements.
The blood was maintained at room temperature and all analyses were carried out within 3 h of blood collection (immediate analysis of all samples was not possible due to the number of samples and time necessary for ROTEM analyses).
Samples were maintained at room temperature and processed within 96 h after blood collection.
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