Blood samples from chicken, cow, crested pigeon (Geophaps lophotes) and sleepy lizard (Tiliqua rugiosa) were spotted onto FTA® paper as normal.
As part of a regional program aimed at detecting the virus, which can cause encephalitis in humans and can be fatal, state officials have been testing dead birds and mosquitoes and taking blood samples from live chickens, which carry the virus but do not get sick from it.
The daily routine of the operational staff includes surveillance of mosquito populations, environment (rainfall, temperature, tide) and presence of West Nile virus (WNV), eastern equine encephalitis virus and St . Louisencephalitis virus through laboratory confirmed seropositive conversion of blood samples from sentinel chickens.
The blood samples from the chicken and cow contained anticoagulant (heparin).
The levels of CA-II in the hemolysate of the chicken were determined using the ELISA system in blood samples from 279 female chickens, ages 1 to 93 weeks, 69 male chickens, ages 3 to 59 weeks and 52 weeks female Araucana-chickens.
The first time point (0 dpi), corresponded to the pooling of 2 ml blood samples from each of the chickens, taken before infection.
For this purpose, blood samples from a total of 384 backyard chickens were collected from wing vein using sterile insulin syringe.
Using blood samples from volunteers, Dr. Stephen Rennard of the University of Nebraska showed that chicken soup inhibited the movement of neutrophils, the most common type of white blood cell that defends against infection.
Recently, HGyV/AGV2 has been detected in Hong Kong in chicken meat for consumption by humans, in human blood samples from donors in France, and in HIV-positive persons and organ transplant recipients in Italy and the United States (3 – 5 ).
