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Arterial blood samples for analyses of electrolytes, blood gases, haematocrit and lactate were taken at the same time points.
Blood samples for analyses of leukocyte receptor expression and complement factors were taken on day 1, 3 and 15.
Blood samples for analyses were drawn from an arterial line according to the protocol in Figure 1.
After injection, serial blood samples for analyses (4 ml) were then obtained over 90 min to calculate the plasma clearance curve.
KKB performed the registration of data, controlled the database, arranged the blood samples for analyses, performed statistical analyses, wrote a draft and completed the manuscript.
At study entry, a physical examination was conducted and each boy and mother provided blood samples for analyses of lead, dioxins, and polychlorinated biphenyls (PCBs).
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Written informed consent was given by the parents of each child, and verbal assent by each child, for collection of a <5 ml venous blood sample for analyses including investigation of malaria parasite DNA.
Blood samples for serum analyses were collected in serum-gel S-Monovette® (Sarstedt AG & Co ., and blood samples for plasma analyses were collected in plasma-gel S-Monovette® with lithium heparin (Sarstedt AG & Co .. Assays for insulin (serum), glucose, lipids and CRP (plasma) were immediately performed in the chemistry laboratory of the University Hospital Inselspital Bern.
Peripheral blood samples for cytokine analyses were obtained from children >2 years of age, centrifuged and serum snap-frozen at −80°C for batch analyses.
Blood samples for pharmacokinetic analyses were collected, and safety and tolerability were assessed.
Liver tissue was harvested on −30, −15, 10, and 30 d, and blood samples for biomarker analyses were obtained more frequently from −30 to 30 DIM.
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