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Participants who consented to donate a blood sample were asked also to collect the urine for 24 hr in a vessel containing 3 g boric acid as preservative.
Breast cancer cases who declined to donate a blood sample were asked to authorise our use of archived paraffin-embedded tissue taken at breast cancer surgery.
Endometrial cancer cases who declined to donate a blood sample were asked to permit to our use of normal tissue from archived paraffin embedded tissue taken at cancer surgery (consisting of, for example, cancer-free lymph nodes, uterine tube or myometrium).
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The 676 women under age 55 who had agreed to be approached about blood sampling were asked to provide a blood sample and 419 (62%) did so.
Participants who were 5 years of age or older at the time of sampling were asked to provide blood at recruitment and at 3 additional time points.
Participants who consented to give a blood sample were also asked to provide a spot morning urine sample, which was stored at –20°C until further analysis.
For the blood samples, participants were asked to fast for at least 12 hours.
After the collection of fasting blood samples, women were asked to drink 75 g of anhydrous glucose in 250 300 ml of water.
At the beginning of the blood sampling, participants were asked when they last ate, and any participant who reported eating or drinking after midnight was considered nonfasting and was asked to return another day when he or she would still receive the incentive.
The subjects were told to avoid foods high in nitrate for 3 days before blood samples were taken, and were asked to fast overnight before sampling blood [ 18].
During the first assessment appointment (approximately 30 minutes), a fasted blood sample was taken and participants were asked to complete a questionnaire pack (See Table 2).
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