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Each blood sample was split between two wells of a 48 well tissue culture dish containing 3.0×104 irradiated STO (Sandoz inbred mouse-derived thioguanine-resistant and ouabain-resistant) feeder cells per well and 0.3 ml of PGC culture medium with or without additional growth factors.
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Human K3EDTA whole blood spiked with working solutions (at LQC and HQC level) were prepared and after spiking spiked sample was split into two aliquots (A and B).
The sample was split into a development and validation sample.
Each RNA sample was split between four different tubes.
The sample is split into five aliquots.
Sampling was split between summer 2010 and winter 2011.
The pre-sample was split into two tubes.
The samples were split into two aliquots.
Subsequently, the samples were split in two.
Surgical samples were split in two fragments.
Samples were split into two 5 L samples for analysis.
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