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Purified DNA from each blood sample was quantified by spectrophotometry, yielding 20 32 ng/μL of DNA.
The presence of CTC in each blood sample was quantified by fluorescence microscopy from 10 images of representative areas (magnification × 100) that were acquired as described above.
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Testosterone in blood samples was quantified by RIA (Beckman-Coulter, Villepinte, France).
Blood samples were quantified for hTERT mRNA expression using real-time polymerase chain reaction.
RNA samples were quantified using spectrophotometry.
Samples were quantified in triplicate.
Samples were quantified using a Bradford assay.
A blood sample was then drawn for tetanus antibody titer and quantified according to a pre-determined cutoff for protection.
The CTCs were easily distinguishable in blood samples and were quantified by fluorescence microscopy on days 4 and 7 after the implantation of the D2A1 FUCCI-expressing cells in the mammary glands.
Cytokine production from unstimulated plasma samples and from stimulated whole blood was quantified with chemiluminescence by using the Immulite automated chemiluminometer (Siemens Healthcare Diagnostics, Deerfield, IL, USA).
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