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DNA was extracted from peripheral venous blood sample using the QIAamp® DNA Mini Kit (Qiagen, West Sussex, UK).
DNA was extracted from each frozen blood sample using the QIAamp blood midi kit (Qiagen, UK) prior to whole genome sequencing.
TC was determined on the same single occasion from a fasting venous blood sample, using the enzyme regent method as our previous study reported [ 7].
Syphilis was detected from a blood sample using the serological test Venereal Disease Research Laboratory (VDRL test, Wiener lab, Rosario, Argentina).
A total of 670 people went on to provide a capillary blood sample using the Bio-Rad point-of-care device after completing the risk questionnaire.
Approximately 2 4 μg of RNA from mononuclear leukocytes was extracted from each blood sample using the RiboPure™-Blood Kit (Applied Biosystems).
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DNA was extracted from the blood samples using the Qiagen DNeasy (Qiagen, Venlo, the Netherlands) and Omega E.Z.N.A Omegaa Bio-Tek, Norcross, GA, USA).
Total genomic DNA was extracted from blood samples using the phenol/chloroform method9.
We determined HbA1c from mouse blood samples using the A1CNow + kit from PTS Diagnostics (Indianapolis, IN).
WES was performed for 38 tumor samples from 18 patients and matched blood samples using the SureSelect Human All Exon Kit Agilent Technologiess) according to the manufacturer's protocols, as previously described (Supplementary Table S1 31,34,35.
We employed blood samples using the same sample tubes collected at one-point arterial blood sampling for the brain QSPECT DTARG method.
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