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Instruments on the machines then identify the amount and the characteristics of chemicals present in a blood sample, using a technique called mass spectrometry.
Genomic DNA was extracted from EDTA-treated peripheral blood sample using a standard procedure.
Blood glucose level was measured immediately before and after the dive series by collecting a capillary blood sample using a commercial device (Accu-Check AVIVA, Roche Diabetes Care Italy S.p.A.Viale G.B. Stucchi 110-20900 MInza MI).
Genomic DNA was isolated from the peripheral blood sample using a phenol-chloroform extraction method [ 21].
DNA was extracted from an EDTA blood sample using a previously described method [ 11].
Genomic DNA used for PCR amplification was extracted from the whole blood sample using a DNA extraction kit (Takara, China).
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N-terminal B-type natriuretic peptide was determined from a venous blood sample using an electrochemiluminescence immunoassay (Elecsys 2010 analyzer, Roche Diagnostics, Basel, Switzerland) [16].
Both protocols included continuous arterial blood sampling using a dedicated on-line sampling system [24].
Genomic DNA was extracted from peripheral blood samples using a salting out method as described by Miller et al. [25].
A randomized cross-over trial design was used comparing capillary blood sampling using a point-of-care device with traditional venous blood sampling.
A hundred blood samples using a fixed type of sample tubes and a fixed radionuclide may be required to set up the robust conversion function.
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