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Each month a blood sample for microscopy and later genotyping was systematically collected.
A blood sample for microscopy (thick and thin film) and a blood spot on filter paper (Whatman grade 3, Whatman, Springfield Mill, USA) for later molecular (PCR diagnosis and microsatellites genotyping) and serological analysis (CSP ELISA) were systematically collected.
Among children providing a blood sample for microscopy diagnosis, 19.1% (95% CI = 16.8 21.8%) slept in a house with ≥ 1 ITN per two occupants (Table 1).
After an interview and clinical examination, survey participants provided a finger prick blood sample for microscopy (thick and thin blood films) and for molecular and serologic tests.
Among children < 5 years old that provided a blood sample for microscopy diagnosis, 81.4% (95% CI = 78.5 84.3%) slept in a house with ≥ 1 ITN (Table 1).
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Baseline malaria screening was conducted in each of the study villages over a three week period from 4th to 22nd August 2008, axillary temperature and finger prick blood sample for malaria microscopy were taken, and study nurses placed in the study villages to actively follow up the health status of the cohort for a period of 22 weeks from 25th August 2008 to 24th January 2009.
A blood sample for a research microscopy slide was taken for all patients.
During surveys, each child was examined by a physician, and a blood sample for hematology, the detection of malaria infection (microscopy), and later, genotyping was collected.
All studies used blood sample for genotyping.
Exosomes can be isolated from blood sample, for in vivo or in vitro studies from specific tissues of the human cell line performed by ultracentrifugation, and then visualized by electron microscopy [ 44].
These included swabs of any discharges for bacteriological culture and typing, viral swabs and heparin blood samples for virological culture, and needle aspirates from enlarged lymph nodes for microscopy.
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