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At each visit, auxiliary temperature was checked, and if found ≥ 37.5°C, a blood sample for microscopic examination (thick and thin blood film) and later, genotyping (Whatman filter paper grade 3) was collected.
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Blood samples for microscopic examination (thick and thin blood film) and for later genotyping and serology on filter paper (Whatman filter paper grade 3) were collected.
Blood samples were collected from all febrile cases using World Health Organization (WHO) standard procedures 27; a single finger prick was used to take a blood sample for the RDT and to prepare thick and thin blood films for microscopic evaluation.
All studies used blood sample for genotyping.
All consenting participants also provided finger-pricked blood samples for Giemsa-stained thick and thin smear microscopic examination.
On each day of follow-up, tympanic temperature was measured by electric thermometer and a finger prick blood sample was used for haemoglobin (Hb) measurement using a Hemocue photometer (Angelholm, Sweden), a microscopic slide, a 50 µL-blood sample for real-time nucleic acid sequence-based amplification (QT-NASBA) and a filter paper sample.
A clinical examination was carried out by a physician (body temperature and spleen size) and a blood sample was collected for microscopic examination (blood smear) and later serological analysis (filter paper).
Primary malaria diagnosis was carried out within 24 hours of blood sampling by microscopic examination of thick and thin blood smears.
Spirochetes were first detected in fresh blood samples by microscopic examination with a QBC kit (Makromed, Johannesburg, South Africa).
Before the introduction of EDTA salts, magnesium salts were used as an anticoagulant for sampling capillary blood for microscopic platelet enumeration, as communicated in a widely respected laboratory handbook in the early 1930s (Lenhartz & Meyer, 1934).
Thus, this platform ensures that virtually all of the CTCs present within a blood sample are deposited onto slides for microscopic analysis, regardless of their size or the expression of specific surface molecules.
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