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Blood meals were processed for 144 An. arabiensis, 53 An. funestus and 7 An.
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No crow blood meals were identified from other habitats.
Blood meals were initially identified by PCR-heteroduplex assays (7 ).
Blood meals were detected in 45 (92%) of 49 bugs, and 43 (96%) of the 45 detected blood meals were successfully cloned (Technical Appendix Table).
However, host-seeking nymphal hard ticks are flat because their last blood meals were months earlier.
blood meals were most frequently identified from sewage treatment plant and storm water catch basin habitats.
On residential properties, 52% of the blood meals were from cardinals.
Blood meals were analyzed according to a method previously described (11 ).
In the present work we sequenced five cDNA libraries constructed from midgut tissue from the sand fly Lutzomyia longipalpis and analyzed the transcripts present following sugar feeding, blood feeding and after the blood meal has been processed and excreted, both in the presence and absence of Leishmania infantum chagasi.
Here we have generated and sequenced five cDNA libraries from the midgut tissue from L. longipalpis; investigated the molecules present during sugar and blood feeding as well as after the blood meal has been processed and excreted, both in the presence and absence of L. infantum chagasi.
Ookinete density inside blood meals is difficult to assess accurately.
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