The strains were grown on blood agar (Biolife, Italy) containing 7%% fresh horse blood and maintained at 37 °C for 48 h.
Resveratrol extracted from grape or Polygonum cuspidatum is a natural antioxidant, which can reduce blood viscosity, maintain the blood flow, and inhibit the platelet aggregation [ 18- 22].
For every 12-point reduction in systolic blood pressure maintained for a decade in 10 patients, one death will be prevented.
Oxygen tension in the circuit blood was maintained between 150 to 200 mmHg (mean 175±± 8 mmHg).
Additionally, glucose concentrations in the blood were maintained at >60 mg/dl and body core temperature was maintained at 37 °C using a heating pad.
Stored blood consistently maintained low levels of nitrite until the end of storage.
For patients exposed to a massive blood loss, maintained coagulation competence is important [ 1– 3].
Hemoperfusion was performed for 2 hours with blood flow maintained at 150 ml/minute.
Oxygen tension in the circuit blood was maintained between 150 and 200 mm Hg. Circuit temperature was maintained at 37°C.
Mosquitoes fed on uninfected blood and maintained as described were used as negative controls (uninfected and oocyst negative).
Two milliliters of EDTA-anticoagulated blood were maintained at room temperature for attempted isolation of Ehrlichia organisms.
