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(PDF 3122 kb) Additional file 13: Selection of reliable reference genes for peripheral blood gene expression analyses.
The bead array system produced by Illumina, Inc. (San Diego, CA, USA) seems to be a cost-effective alternative, hopefully as sensitive and reliable without the need for globin reduction, but this should be evaluated before conducting blood gene expression analyses in future studies of the NOWAC cohort.
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Relevant to this study are studies using blood cell gene expression analyses to evaluate environmental chemical exposures [ 5- 7].
b44 individuals were included for the dura and blood gene expression individual clustering analyses; 40 individuals were included for all other analyses.
In addition, blood gene expression and candidate SNP analyses were conducted.
For gene expression analyses, blood samples of 2.5 mL were collected from each subject into PAXgene Blood RNA vacutainer tubes (PreAnalytix; Qiagen, Hilden, Germany), a system that accounts for immediate ex vivo preservation of blood RNA.
Each participant had minimally 5 ml PAXgene blood collected for RNA isolation and gene expression analyses.
The informed consent formula explicitly mentions that the blood samples can be used for gene expression analyses as well as large-scale genotyping.
Gene expression analyses of the genes mentioned above were performed by quantitative PCR.
Each participant had minimally 5 ml of PAXgene blood collected for leucocyte RNA isolation and gene expression analyses.
The population-based NOWAC postgenome cohort study provides opportunities for conducting nested case-control studies using gene expression analyses of whole blood [ 23].
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