PBMCs were isolated by Ficoll-hypaque density gradient centrifugation, and viral DNA was extracted using QIAamp blood extraction kit (QIAGEN).
Parasite culture in erythrocytes was performed using standard methods [ 42], and genomic DNA (gDNA) was extracted using the QIAquick whole blood extraction kit (QIAGEN) and eluted into 30 μl of a low salt buffer.
Genomic DNA was extracted with the Qiagen Midi DNA blood extraction kit (Qiagen, spin protocol UK catalog number 51183) with 2 ml of whole blood including the recommended proteinase K and RNase A digestions.
Genomic DNA was extracted from cell samples using the Qiagen DNeasy Blood Extraction Kit (Qiagen, Gaithersburg, MD, USA).
RNA was extracted ex-vivo from blood using the PAXgene RNA blood extraction kit (PreAnalytix, Switzerland) per the manufacturer's instructions and concentrated by ethanol precipitation.
Fresh tissues from individual fish were taken and DNA was extracted using a DNeasy Qiagen kit, following the tissue and blood extraction protocol provided along with the kit.
DNA was extracted using standard methods and RNA with the RiboPure™ Blood extraction kit (Applied Biosystems/Ambion, Austin, TX, USA).
To this end, the most depraved techniques – electric shocks, rape, the forced eating of excrement, medical experimentation, flaying, and lethal blood extraction – were employed.
The evaluation was performed during blood extraction, vein canalization, and heel puncture.
We analyzed the influence of clinical factors and timing of blood extraction on serum concentrations of cholesterol and triglycerides in exocrine pancreatic cancer (EPC).
The blood extraction practices described in "In the Amazon, Giving Blood but Getting Nothing" (front page, June 20) amount to taking advantage of unsophisticated people in the developing world for the benefit and material gain of individuals and companies in the developed West.
