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As previously published (Schuh-Huerta et al. 2012), genomic DNA was extracted and purified from white blood cells using the QIAamp DNA Blood Maxi Kit (Qiagen, Venlo, The Netherlands) according to the manufacturer's instructions.
Next, we determined whether trypsins that are known to activate protease-activated receptors (PARs) on cells could activate blood cells using the thrombocyte aggregation assay.
DNA was extracted from the collected white blood cells using the perfect pure DNA blood kit (5prime.com) following the manufacturer's protocol.
The aim of the work reported here was to elucidate the mechanism of a transient current response encountered during patch clamping of human red blood cells using the cell-attached configuration.
As the catalog of our coding exons, the "exome", is improving in definition, we took the opportunity to analyze the expression of over 1 million known or predicted human exons in immature hematopoietic progenitor cells & hematopoietic stem cells (HSPC) and mature whole blood cells using the GeneChip Human Exon 1.0 ST (Affymetrix) microarray.
Genomic DNA was prepared from white blood cells using the salting-out method [ 18].
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Specific immunoreactions were obtained for nerve fibers and white blood cells using both the polyclonal and monoclonal antibodies, whilst specific immunoreactions in the blood vessel walls were only obtained with the polyclonal antiserum.
After irradiating peripheral blood cells using 2 Gy, the count of γH2AX foci formation reached its maximum after 30 minutes and declined over the next hours.
Lead levels were measured in red blood cells using adaptations of the methods described by Miller et al. [ 16] and Parsons and Slavin [ 17].
The total number of peripheral blood leukocytes was determined by lysing the red blood cells using 3% acetic acid solution with the aid of a Neubauer hemocytometer.
We extracted DNA for sexing from the frozen blood cells using a Blood Mini kit (A&A Biotechnology, Gdynia, Poland).
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