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Researchers have known for more than a decade that HIV slips into white blood cells using a receptor found on their surface known as CD4, but it appeared to need some other coreceptor, too.
After removal of host white blood cells using a CF11 column, 2 ml of packed infected Red blood cells (IRBC) were divided as follows: 1 ml was cryopreserved in glycerolyte, 200 µl spotted onto a filter paper and 800 µl was used for the in vitro drug susceptibility assay.
Genomic DNA was prepared from white blood cells using a standard procedure.
DNA was extracted from white blood cells using a standard phenol chloroform extraction procedure.
After centrifugation, plasma was separated from red blood cells using a disposable Pasteur pipette.
DNA was extracted from blood cells using a Whole Blood DNA Isolation Kit (Dojindo Molecular Technologies , Inc.
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We then checked blood cells using an in vitro whole-blood culture model.
DNA was extracted from blood cells using proteinase K treatment followed by a salting [ 37], or a phenol-chloroform purification method [ 38].
DNA was extracted from peripheral blood cells using standard procedures.
Genomic DNA was extracted from peripheral blood mononuclear cells using a QIAamp DNA blood mini kit (Qiagen).
Autologous CD4+ T cells used for B/T cell coculture were purified from blood mononuclear cells using a human CD4+ T Cell Enrichment kit (StemCell Technologies).
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