DNA was extracted from peripheral blood cells using standard procedures.
P. falciparum was cultivated in human A Rh+ red blood cells using RPMI 1640 medium (Sigma, India) supplemented with AB Rh+ serum (10%), 5% sodium bicarbonate (Sigma, India) and 40 μg/mL of gentamycin sulphate 17 (Sigma, India).
In this study, we present a comprehensive finite element-based simulation of acoustic separation of platelets, red blood cells and white blood cells, using standing surface acoustic waves (SSAWs).
Genomic DNA was isolated from peripheral blood cells, using standard methods.
Moreover, GFP-expressing transgenic parasites in blood stages can be readily differentiated from other blood cells using flow cytometry.
Next, we determined whether trypsins that are known to activate protease-activated receptors (PARs) on cells could activate blood cells using the thrombocyte aggregation assay.
DNA was extracted from the collected white blood cells using the perfect pure DNA blood kit (5prime.com) following the manufacturer's protocol.
Genomic DNAs from normal and vagal hyperreactive rabbits were isolated from peripheral blood cells using Qiagen technology (GmbH, Hilden, Germany) and quantified by spectrophotometry.
Briefly, marrow cells prepared from the CD45.1 mice were first depleted of red blood cells using ACK solution (0.15 M ammonium and 0.01 M potassium carbonate).
The aim of the work reported here was to elucidate the mechanism of a transient current response encountered during patch clamping of human red blood cells using the cell-attached configuration.
To determine whether all of the transgenic parasites of the blood stages showed green fluorescence and could be readily differentiated from other blood cells using flow cytometry, blood samples were collected daily from PfMSP1-19Pb8.7-infected mice.
