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The program enables mothers who do not deliver at a CCBB cord blood collection site to donate their baby's cord blood by using a kit.
Genomic DNA was extracted from peripheral blood by using a commercially available kit (Macherey Nagel, Düren, Germany), according to manufacturers' instructions.
Genomic DNA from bearded collies and giant schnauzers was extracted from 200 µl EDTA blood by using a standard salt extraction protocol, the Qiagen QIAamp DNA Blood Mini Kit (Qiagen, Valencia, CA).
Ticks were fed infected blood by using a Hemotek membrane-feeding system (Discovery Workshops, Accrington, UK).
Genomic DNA was isolated from peripheral blood by using a standard phenol-chloroform extraction method [ 12].
DNA was extracted from whole blood by using a standard phenol/chloroform protocol.
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A similar device has been developed to separate serum from whole blood by using an egg beater.
The strongest evidence that ticks might be competent vectors for bartonellae was reported in a recent study in which I. ricinus ticks were infected with B. henselae in spiked (artificially infected) ovine blood by using an artificial feeding system (23 ).
Physiological parameters (i.e., arterial pH, PaCO2, PaO2) were analyzed with 50 μl of femoral artery blood by using an AVL OPTI 3 Blood Gas Analyzer (AVL Co., Plymouth, MI, USA) and monitored at three time points, that is, 5 min before MCAo, immediately after MCAo and 45 min after MCAo (Supplementary Figure 11a).
Genomic DNA was extracted from the buffy coat fraction of each blood sample by using a Blood Mini Kit (Qiagen, Valencia, CA) according to the manufacturer's instructions.
DNA was extracted from anticoagulated blood samples by using a Blood DNA Extraction Kit (QIAGEN, Germantown, MD, USA) according to the manufacturer's instructions.
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