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The blocks were transferred into a microcentrifuge tube, resuspended in 0.5 mL 2% glutaraldehyde and stored for 1 2 h at room temperature.
Subsequently, the blocks were transferred into 10 mM Tris/HCl buffer pH 8.0 containing 0.5 mM EDTA, 1% (v/v) sarkosyl and fresh proteinase K at 2 mg/ml and incubated for 48 h at 37°C to free the nucleic acids.
Solidified blocks were transferred into 50% ethanol for 1 h and then 80% ethanol before embedding in paraffin.
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The hollow portions of the hollow fibres in the resin block were filled with this solution, the block was transferred into a humidified hermetically sealed glass vessel, and the content of the vessel was allowed to polymerize at 70°C for 3 h.
The tissue blocks were transferred to a recipient "master" block using a Tissue Microarrayer.
To block endogenous peroxidases, slices were transferred into phosphate-buffered 3% H2O2 for 30 minutes.
Samples were transferred into nutrition blocks containing normal garden soil for 30 days.
The U6 promoter-shRNA cassettes were transferred into the pLenti6-BLOCK-iT-DEST vector.
On average, 4 tissue cylinders of 0.6 mm diameter were obtained and were transferred into a recipient paraffin block using a manual tissue arrayer (Alphelys, Plaisir, France).
Separated proteins were transferred into a nitrocellulose membrane, and blocked with 2.5% BSA in TBST.
Proteins were transferred into a PVDF membrane (90 min; 12V), blocked (3% BSA) and incubated overnight with anti-α-gal IgG2b mAb (1 μg/ml; 20 ml).
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