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The blocks were subjected to tension in two orthogonal directions, which were perpendicular to the extrusion direction.
The blocks were subjected to compression test and water absorption test according to Indian Standard (IS) specifications.
Next, the dentine blocks were subjected to sterilization by autoclave (LTE®, Oldham, Lancashire, UK) for 20 minutes at 121°C.
The thin sections cut from these cell blocks were subjected to immunofluorescence staining and immunohistochemical staining (Fig. 2C and D).
At each institution, 4-μm-thick sections cut from routinely processed formalin-fixed, paraffin-embedded tissue blocks were subjected to immunohistochemistry and FISH.
The dissected, protein-containing gel blocks were subjected to trypsin treatment (0.2 μg, trypsin in 25 mm NH4HCO3 buffer at 37°C overnight).
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To better understand pathogenesis of cardiovascular SLE and provide biomarkers for prompt diagnosis, auto-antigens involved in congenital heart block were subjected to proteolytic cleavage with and without sera of SLE patients, followed by MALDI-TOF-TOF identification of antibody-protected sites (epitopes) [ 19].
When the proportion of cancer cells in a tissue section was greater than 80%, the frozen block was subjected to RNA extraction.
One slide of each TMA block was subjected to hybridization reactions.
The FFPE blocks were subject to microdissection into IDC and normal components as described previously [ 19].
Three HEB100 structural profiles were embedded in concrete blocks and were subjected to statically increasing pushout loads, till the signs of bond slip between concrete and steel were observed, when the specimens were considered failed.
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