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Blocks were sectioned in the sagittal plane.
Blocks were sectioned in 5 μm thickness using a Leica RM2265 microtome.
The paraffin embedded blocks were sectioned in 3-10μm slices and mounted on adhesion microscope glass slides (SuperFrost plus, Menzel J1800AMNZ).
Tissue blocks were sectioned in the sagittal plane of the fusion mass included the transverse processes at 5 μm and stained with hematoxylin and eosin and Masson trichrome methods.
For ultrastructural evaluation, resin blocks were sectioned in the transverse plane at 100 nm intervals, stained sequentially with uranyl acetate and lead citrate and examined with a Hitachi® 7500 transmission electron microscope at 100 kV.
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Selected paraffin blocks were sectioned (5μm sections) and mounted in microscope slides.
For FFPE samples, the selected blocks were sectioned at 10 μm thickness in an RNase-free environment.
Cancer tissues in paraffin blocks were sectioned.
After solidification, paraffin blocks were sectioned serially at 8 μm thickness in longitudinal or transverse orientation.
Forebrain blocks for immunostaining were sectioned in the coronal plane on a vibratome (VT 1200S, Leica, Wetzlar, Germany) at 40 µm thickness.
The intestinal tissue block was sectioned at 5 μm in cryostat microtome at −20 °C, with special attention given to preserve the fragile structures in the block.
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