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Security was tight in the US capital and 100 square blocks were sealed to traffic.
Coal blocks were sealed to preserve natural conditions.
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Edges were sealed to prevent drying.
After construction, tissue microarray blocks were sealed with paraffin and stored at 4°C.
Completed array blocks were sealed for 10 min in a 60°C oven.
The culture blocks were sealed with an air permeable seal (Excel Scientific, Wrightwood, CA) and shaked at 37°C for 20 hours with the speed of 300 rpm.
The constructed TMA blocks were sealed with paraffin, and 5- μm-thick slides were cut from the TMA blocks for immunohistochemical staining.
Blocks were sealed with an air-pore membrane and incubated in an Infors Multitron II incubator at 37°C, 5 % CO2, 40%% humidity at 400 rpm.
Several gardens overflow with rubbish, and the ground floor of one high-rise block is sealed off with metal grates to keep out vandals.Yet dotted among the half-century-old buildings are a few bright new homes, with large parking spaces, carefully tended gardens and solar panels on the roofs.
The block was sealed with a sealing mat (Thermo Scientific) and frozen for at least 1 hour at −80°C.
The block was cooled on ice-water, 150 μL of 6 M ammonium acetate was added and the block was sealed with PCR film.
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