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Surgical blocks were retrieved for pathological scoring in 26 cases.
Archived formalin-fixed paraffin-embedded (FFPE) tumor blocks were retrieved for TMA construction.
From the 'low-risk' patient group (negative lymph nodes and tumour size ⩽3 cm) in this trial, a total of 808 FFPE tumour blocks were retrieved for molecular analysis.
Formalin-fixed paraffin-embedded tissue blocks were retrieved for each patient and used to construct a tissue microarray (TMA; Beecher MTA1 machine (Beecher Instruments Inc., Silver Springs, MD, USA); Alphelys TMA designer, Alphelys, Plaisir, France).
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Hematoxylin and eosin stained slides corresponding to formalin-fixed paraffin embedded (FFPE) tissue blocks are retrieved for all participants for whom tissue is available.
The routine haematoxylin and eosin slides of the resected tumour specimens along with corresponding paraffin blocks were retrieved from the pathology archive for all patients in our study group.
For the Scottish group, donor blocks were retrieved from patients recruited into seven clinical trials and TMAs were constructed separately for each trial.
Briefly, tissue blocks were retrieved from the Research Group Human Genetics, Department of Biomedicine, University of Basel.
Formalin-fixed, paraffin-embedded blocks were retrieved from the Department of Pathology.
Blocks were retrieved from the BioRepository Center of the NYU School of Medicine.
For the current analysis, following approval by central ethics committee, tissue blocks were retrieved from 322 cases (85.8%).
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