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After 1, 4 and 9 weeks of submerged healing, dissected blocks were processed for immunohistochemical (osteocalcin) and histomorphometrical analysis (residual defect length, new bone-implant contact, area of new bone fill, percentage of osseointegrated bone-graft particles).
Three serial sections (5 μm thick) per experiment from paraffin-embedded blocks were processed for hematoxylin and eosin (H&E) for morphological evaluation and immunohistochemical analysis.
Five-micrometer sections from formalin-fixed, paraffin-embedded blocks were processed for immunostaining using the Discovery XT automated staining system (Ventana, Tucon AZ).
The obtained tissue blocks were processed for paraffin embedding and subsequent staining by hematoxylin and eosin.
Skin blocks were processed for EM and ultrastructural morphometry of Remak axons done.
Sections (5 μm) from the paraffin tissue blocks were processed for immunohistochemical analysis.
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Frozen sections (5 μm) were stained with haematoxylin and eosin (H & E) to verify OSCC and approximately 0.1 g of the tumour block was processed for RNA extraction using the TRIZOL Reagent (GIBCO BRL, Grand Island, NE, USA).
Tissue blocks were processed routinely for paraffin sections.
Sections from these paraffin blocks were processed and stained for LC3 and this revealed a marked and statistically significant increase (p < 0.01) in LC3-positive puncta in EBSS-treated cells representing the accumulation of autophagosomes associated with induction of autophagy (Fig. 5A).
A total of 80 separate osteosarcoma tissue blocks were processed using immunostaining and analyzed for APE1, FGF2, FGER3 and CD34.
Formalin-fixed and paraffin-embedded cell blocks were processed to obtain seriate tissue sections for both morphologic analysis and phenotypic studies.
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