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Six sets of mesodiencephalic blocks were prepared for carbocyanine dye (DiI, Sigma, 468495) analysis.
Bone and cartilage tissue blocks were prepared for histological analysis.
Paraffin bees wax tissue blocks were prepared for sectioning at thickness of 4 μm by sliding microtome.
Formalin-fixed, paraffin-embedded tissue blocks were prepared for immunohistochemistry at the Paterson Institute for Cancer Research PICRR).
The representative tumor areas were selected and marked on H&E-stained slides, and the corresponding paraffin blocks were prepared for the construction of a tissue microarray (TMA).
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A single formalin-fixed, paraffin-embedded block was prepared for each patient's tumour tissue and normal surrounding parenchyma.
A haematoxylin/eosin-stained section of each block was prepared for central confirmation of the histological diagnosis and determination of the histological type and grade [ 23].
Block is prepared for carving.
Tissue blocks were prepared and evaluated for histopathological changes.
A total of three TMA blocks were prepared and sectioned for in situ hybridization analysis of miRNA expression.
A total of five TMA blocks were prepared and sectioned for in situ hybridization and immunohistochemical analysis.
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